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CBSE Class 9 Biology Chapter 5 The Fundamental Unit of Life — 20 MCQs with Answers

CBSE Class 9 Biology Chapter 5 introduces the cell as the fundamental unit of life—every living organism is made of one or more cells. From Robert Hooke's 1665 discovery to modern cell theory, this chapter covers cell structure, types (prokaryotic and eukaryotic), and key organelles like the nucleus, mitochondria, and chloroplasts. The following 20 MCQs test your grasp of NCERT content, mixing straightforward recall with application and higher-order thinking questions. Practise them to strengthen conceptual clarity and boost confidence for school tests and board exams.

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Key takeaways

  • The Cell Theory states all living organisms are made of cells and all cells come from pre-existing cells—Schleiden, Schwann, and Virchow.
  • Prokaryotic cells (bacteria) lack a true nucleus and membrane-bound organelles; eukaryotic cells (plants, animals) have both.
  • The plasma membrane is selectively permeable, regulating what enters and exits the cell via the fluid mosaic model.
  • Mitochondria generate ATP through aerobic respiration and are called the 'powerhouse of the cell'.
  • Chloroplasts in plant cells carry out photosynthesis, converting light energy into chemical energy stored as glucose.
  • The nucleus controls all cellular activities by storing DNA and directing protein synthesis through mRNA.
  • Plant cells have a rigid cellulose cell wall and a large central vacuole; animal cells lack both these structures.

Cell Discovery and Cell Theory — MCQs 1–4

Understanding how cells were discovered and the development of Cell Theory is fundamental. Robert Hooke coined the term 'cell' in 1665 while observing cork under a microscope. Matthias Schleiden and Theodor Schwann proposed that all living organisms are made of cells. Rudolf Virchow later added that all cells arise from pre-existing cells. These historical milestones form the basis of modern cell biology and appear regularly in CBSE exams as factual recall or assertion-reason questions. The following MCQs test your knowledge of scientists, their contributions, and the tenets of Cell Theory.
  • Q1. Who coined the term 'cell' and in which year? (A) Robert Brown, 1831 (B) Robert Hooke, 1665 (C) Matthias Schleiden, 1838 (D) Anton van Leeuwenhoek, 1674 | Answer: (B) Robert Hooke, 1665. Reason: Hooke observed cork slices under a microscope and named the box-like structures 'cells'.
  • Q2. Which scientist concluded that all plants are made of cells? (A) Theodor Schwann (B) Rudolf Virchow (C) Matthias Schleiden (D) Robert Hooke | Answer: (C) Matthias Schleiden. Reason: Schleiden in 1838 proposed that all plant tissues are composed of cells.
  • Q3. The statement 'Omnis cellula e cellula' (all cells from cells) was given by: (A) Robert Hooke (B) Matthias Schleiden (C) Rudolf Virchow (D) Theodor Schwann | Answer: (C) Rudolf Virchow. Reason: Virchow in 1855 stated that all cells arise from pre-existing cells, completing Cell Theory.
  • Q4. Which of the following is NOT a tenet of Cell Theory? (A) All living organisms are composed of cells (B) The cell is the basic unit of life (C) All cells arise from pre-existing cells (D) All cells contain chloroplasts | Answer: (D) All cells contain chloroplasts. Reason: Only plant cells and some protists have chloroplasts; animal cells do not.

Prokaryotic vs Eukaryotic Cells — MCQs 5–8

Cells are broadly classified into prokaryotic and eukaryotic based on the presence of a membrane-bound nucleus. Prokaryotic cells (bacteria, cyanobacteria) lack a true nucleus and have DNA in a nucleoid region. They are small, simple, and lack membrane-bound organelles. Eukaryotic cells (found in animals, plants, fungi, protists) have a well-defined nucleus enclosed by a nuclear membrane and contain organelles like mitochondria, ER, and Golgi apparatus. Understanding this distinction is crucial for NCERT-based MCQs and helps you identify cell types in diagrams. The next four questions focus on structural and functional differences.
  • Q5. Which of the following organisms has prokaryotic cells? (A) Amoeba (B) Yeast (C) *E. coli* (D) Spirogyra | Answer: (C) *E. coli*. Reason: *E. coli* is a bacterium with no true nucleus; the others are eukaryotes.
  • Q6. The genetic material in a prokaryotic cell is located in: (A) Nucleus (B) Nucleoid (C) Mitochondria (D) Ribosome | Answer: (B) Nucleoid. Reason: Prokaryotes lack a membrane-bound nucleus; DNA floats freely in the nucleoid region.
  • Q7. Which of these organelles is found ONLY in eukaryotic cells? (A) Ribosome (B) Cell membrane (C) Mitochondria (D) Cell wall | Answer: (C) Mitochondria. Reason: Ribosomes and cell membranes are in both; cell walls in bacteria too, but mitochondria are eukaryote-specific.
  • Q8. Assertion: Eukaryotic cells are larger and more complex than prokaryotic cells. Reason: Eukaryotic cells have membrane-bound organelles that allow compartmentalization. (A) Both true, Reason explains Assertion (B) Both true, Reason does not explain (C) Assertion true, Reason false (D) Both false | Answer: (A) Both true, Reason explains Assertion. Reason: Compartmentalization enables greater size and complexity.

Plasma Membrane and Diffusion — MCQs 9–12

The plasma membrane (cell membrane) is a selectively permeable barrier made of a lipid bilayer with embedded proteins—the Fluid Mosaic Model. It regulates the entry and exit of substances, maintaining homeostasis. Small, non-polar molecules like oxygen and carbon dioxide pass through by diffusion, while larger or charged molecules require protein channels or active transport. Understanding selective permeability and the structure of the membrane is essential. These MCQs test your grasp of membrane composition, function, and the concept of diffusion across the membrane.
  • Q9. The plasma membrane is mainly composed of: (A) Cellulose and proteins (B) Lipids and proteins (C) Nucleic acids and carbohydrates (D) Lipids and nucleic acids | Answer: (B) Lipids and proteins. Reason: The lipid bilayer forms the structure; proteins are embedded for transport and signalling.
  • Q10. The Fluid Mosaic Model describes the structure of: (A) Cell wall (B) Nucleus (C) Plasma membrane (D) Chromosome | Answer: (C) Plasma membrane. Reason: The model shows lipids as a 'fluid' layer with proteins as a 'mosaic' of embedded molecules.
  • Q11. Which substance can pass through the plasma membrane by simple diffusion? (A) Glucose (B) Sodium ions (C) Oxygen (D) Proteins | Answer: (C) Oxygen. Reason: Oxygen is small and non-polar, diffusing freely through the lipid bilayer; glucose and ions need transport proteins.
  • Q12. The plasma membrane is called selectively permeable because: (A) It allows all substances to pass freely (B) It allows only water to pass (C) It allows some substances to pass while blocking others (D) It does not allow any substance to pass | Answer: (C) It allows some substances to pass while blocking others. Reason: Selective permeability maintains the cell's internal environment by controlling what enters and exits.

Cell Wall, Nucleus, and Chromosomes — MCQs 13–16

The cell wall is a rigid, non-living layer outside the plasma membrane in plant cells, fungi, and bacteria. In plants, it is made of cellulose and provides structural support, shape, and protection. The nucleus is the control centre of eukaryotic cells, enclosed by a nuclear envelope with pores. It contains chromosomes—structures made of DNA and protein that carry genetic information. The nucleolus inside the nucleus synthesizes ribosomal RNA. These structures are central to understanding cell organization and function. The following MCQs cover cell wall composition, nuclear structure, and the role of chromosomes.
  • Q13. The plant cell wall is primarily made of: (A) Peptidoglycan (B) Chitin (C) Cellulose (D) Lipids | Answer: (C) Cellulose. Reason: Cellulose is a tough carbohydrate that forms the rigid plant cell wall; chitin is in fungi, peptidoglycan in bacteria.
  • Q14. The nuclear envelope has pores that allow: (A) Free movement of all substances (B) Selective exchange of materials between nucleus and cytoplasm (C) Entry of cytoplasm into the nucleus (D) Exit of nucleus into cytoplasm | Answer: (B) Selective exchange of materials between nucleus and cytoplasm. Reason: Nuclear pores regulate passage of RNA and proteins, maintaining nuclear integrity.
  • Q15. Chromosomes are made of: (A) DNA only (B) RNA and proteins (C) DNA and proteins (D) Lipids and carbohydrates | Answer: (C) DNA and proteins. Reason: Chromosomes consist of chromatin—DNA tightly wound around histone proteins.
  • Q16. The nucleolus is the site of synthesis of: (A) DNA (B) mRNA (C) Ribosomal RNA (D) Lipids | Answer: (C) Ribosomal RNA. Reason: The nucleolus produces ribosomal RNA, which is assembled into ribosomes for protein synthesis.

Mitochondria, Chloroplasts, and Energy — MCQs 17–20

Mitochondria are double-membrane organelles found in nearly all eukaryotic cells. They perform aerobic respiration, converting glucose and oxygen into ATP (the cell's energy currency) and releasing carbon dioxide and water. They have their own DNA and ribosomes, suggesting an evolutionary origin from ancient bacteria. Chloroplasts, found only in plant cells and some protists, contain chlorophyll and carry out photosynthesis—converting light energy, carbon dioxide, and water into glucose and oxygen. Both are energy-transforming organelles and crucial for life on Earth. These MCQs test your understanding of their structure, function, and evolutionary significance.
  • Q17. Mitochondria are known as the 'powerhouse of the cell' because they: (A) Store food (B) Synthesize proteins (C) Produce ATP through aerobic respiration (D) Digest waste materials | Answer: (C) Produce ATP through aerobic respiration. Reason: Mitochondria break down glucose with oxygen to release energy stored in ATP molecules.
  • Q18. Chloroplasts are found in: (A) All eukaryotic cells (B) Animal cells only (C) Plant cells and some protists (D) Prokaryotic cells | Answer: (C) Plant cells and some protists. Reason: Only photosynthetic organisms have chloroplasts; animal cells lack them.
  • Q19. The process of photosynthesis occurs in: (A) Mitochondria (B) Nucleus (C) Chloroplasts (D) Ribosomes | Answer: (C) Chloroplasts. Reason: Chloroplasts contain chlorophyll, which captures light energy to drive photosynthesis.
  • Q20. Both mitochondria and chloroplasts have their own DNA and ribosomes. This supports the theory that: (A) They were once free-living prokaryotes engulfed by early eukaryotic cells (B) They are formed by the nucleus (C) They can survive outside the cell (D) They are non-living organelles | Answer: (A) They were once free-living prokaryotes engulfed by early eukaryotic cells. Reason: The endosymbiotic theory explains their double membranes and independent genetic material.

How to Attempt MCQs in the CBSE Biology Paper

CBSE Class 9 Biology exams typically include 10–15 MCQs worth 1 mark each in the objective section. Strategic MCQ-solving can save time and improve accuracy. First, read each question carefully—watch for keywords like 'NOT', 'ONLY', or 'EXCEPT' that reverse meaning. Eliminate obviously wrong options to narrow your choice. If unsure, recall NCERT diagrams or definitions verbatim—CBSE strictly follows NCERT terminology. For assertion-reason questions, verify both statements independently, then check if the reason logically explains the assertion. Don't spend more than 30–40 seconds per MCQ; if stuck, mark it and return later. Cross-checking answers in the last 5 minutes can catch silly mistakes. Practising 50–100 MCQs from each chapter builds speed and confidence. CBSETUTOR.ai offers unlimited chapter-wise MCQ practice with instant explanations, photo-upload doubt solving, and personalized error tracking—all at a flat ₹999/month for Classes 6–12 with a 3-day free trial. Regular MCQ practice transforms exam anxiety into assured performance.
  • Read the question stem twice to spot tricky words like 'incorrect' or 'least likely'.
  • Eliminate two obviously wrong options first, then choose between the remaining two using NCERT facts.
  • For diagram-based MCQs, label parts mentally before looking at options—helps avoid confusion.
  • In assertion-reason MCQs, check: (i) Is A true? (ii) Is R true? (iii) Does R explain A? Then pick the correct code.
  • Never leave MCQs blank—there is no negative marking in CBSE Class 9; guess intelligently if needed.
  • Revise marked answers in the last 5 minutes—many students catch misread options or calculation slips.

Frequently asked questions

How many MCQs from Chapter 5 can I expect in the CBSE Class 9 Biology exam?+
Typically 2–3 MCQs (1 mark each) appear from this chapter in the objective section of the CBSE term exam. The chapter also appears in 2–3 mark short-answer questions and diagram-based questions, so thorough MCQ practice strengthens overall chapter command.
What is the difference between prokaryotic and eukaryotic cells in simple terms?+
Prokaryotic cells (like bacteria) have no membrane-bound nucleus—DNA floats freely in the nucleoid. Eukaryotic cells (plants, animals, fungi) have a true nucleus enclosed by a nuclear membrane and contain organelles like mitochondria and ER. Prokaryotes are smaller and simpler.
Why is the plasma membrane called selectively permeable?+
The plasma membrane allows certain substances (oxygen, carbon dioxide, water) to pass through freely while blocking others (large proteins, ions) or requiring special transport proteins. This selective control maintains the cell's internal environment and is vital for homeostasis.
Do animal cells have a cell wall?+
No. Animal cells lack a cell wall; they have only a flexible plasma membrane. Plant cells, fungi, and bacteria have rigid cell walls. This is a common CBSE MCQ—remember, animal cells have no cell wall or chloroplasts.
What is the function of mitochondria in one sentence?+
Mitochondria perform aerobic respiration, breaking down glucose with oxygen to produce ATP (energy currency) for cellular activities. That is why they are called the 'powerhouse of the cell'.
Which organelle is responsible for photosynthesis?+
Chloroplasts carry out photosynthesis in plant cells and some algae. They contain the green pigment chlorophyll that captures light energy and converts carbon dioxide and water into glucose and oxygen.
What is the role of the nucleus in a cell?+
The nucleus controls all cellular activities by storing DNA (genetic material) and directing protein synthesis. It sends instructions via mRNA to ribosomes in the cytoplasm, determining which proteins are made and when.
How should I prepare MCQs from this chapter for the CBSE exam?+
Read NCERT thoroughly, underline key terms (nucleoid, chromatin, cristae, selectively permeable), and practice at least 30–50 MCQs mixing recall, application, and assertion-reason types. Use NCERT back exercises and previous year papers. CBSETUTOR.ai offers unlimited MCQ practice with instant feedback at ₹999/month for all classes.
Are there any assertion-reason MCQs in CBSE Class 9 Biology?+
Yes. CBSE now includes assertion-reason MCQs where you must decide if both statements are true and whether the reason correctly explains the assertion. Practice these formats separately—they require careful reading and logical reasoning, not just recall.
Can I score full marks in the MCQ section of Biology?+
Absolutely. MCQs are direct and NCERT-based. Revise definitions, diagrams, and scientist contributions verbatim. Practice 100+ MCQs from reliable sources, time yourself, and review mistakes. With consistent practice, full marks in the objective section is achievable.

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